Sixty-Six Years of Experimentation
Lord Liu Chun spent two years discussing and planning the experimentation with his colleagues, three hundred medical doctors who had been assigned to him by the Emperor. Lord Liu also received the assistance of several thousand soldiers from the Imperial army. All his research was funded by the Emperor.
He collected 2,000 years of medical history, classified 2,175 diseases, and listed more than 5,000 types of herbs and ingredients and 61,739 recipes and formulas. His first step was to organize the diseases in sixteen classifications ( 证候群 ). He created two more categories based on the lack of hunger and appetite and the proper consumption of food. He divided his staff into eighteen teams to study the eighteen classifications. These experiments were conducted repeatedly over a span of several years.
At the same time, Lord Liu Chun tested each of the 5,611 herbs to identify the best applications. The experiments were conducted over a period of sixty-six years and required the participation of hundreds of thousands of death row inmates.
The results were kept secret by his family and the Imperial Physicians. Only in recent years have these secrets been revealed by his twenty-fourth generation descendant, Dr. Liu Hong Zhang.
In 1409 there were five thousand death row inmates in Nanjing and Lord Liu began eighteen simultaneous experiments. All five thousand prisoners were used; three thousand six hundred people (eighteen groups of two hundred people each) were initially assigned for research. In the process of inducing illness in patients, some patients died prematurely and were replaced. Additionally, some patients did not become sick because of genetic predispositions, and those patients were also replaced. After a prisoner completed his trials, he was pardoned and released on the northeastern border of China. Every year, additional death row inmates were added. Each area of experimentation involved many years of trial and many thousands of prisoners were used. Some prisoners participated in studies of ingredients that were not effective and they were not cured by their test treatment. Lord Liu ordered that they should be returned to health when the most effective cure for their illness was identified. Subsequently, they were pardoned and released.
Subject of Research
1. Cancer—shark gallbladder 、 bovine Achilles tendon
2. Diabetes
3. Lethargy and physical weakness
4. Energy in the stomach (hunger)
5. Influenza
6. Erectile dysfunction
7. Hepatitis
8. Cholera
9. Bruise and injuries
10. Epilepsy, schizophrenia, drug addiction
11. Chronic enteritis, gastric ulcer
12. Aplastic anemia, endometrorrhagia
13. Preventative food therapy
14. Nephritis, cardiomyopathy
15. Acne, appendicitis
16. Arteriosclerosis, coronary heart disease, apoplexy, stroke
17. Ten points to enhancement of life
18. Rheumatoid arthritis, lupus,hysteromyoma
19. Bronchitis
20. Pregnancy
21. Problems with alcohol
22. Advantages of meat soup and the disadvantage of eating meat
23. Study of human Yin and Yang energy
24. Methods of pain relief
25a. Study of chemical calcium tablets
25b. Negative side effects of chemical calcium tablets
26. Treatment of patients with multiple diseases
27. Aphrodisiac drugs and early death
28. Water intake
29. Study of 5,611 herbs
a. Research on drugs
b. Drug therapeutic effect
30. Study of acu-points and acu-meridians
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Shark Gallbladder
Historical Chinese Research
Nine hundred years ago, in the twelfth century, an ancestor of Lord Liu Chun, Dr. Liu Wansu ( 刘元素 ), found that shark gallbladders could be used in the treatment of cancer. Lord Liu Chun used shark gallbladders in the treatment for cancer for the Empress Yong Le. The treatment prolonged her life by seven years, and she died in 1407.
Fresh shark gallbladders, however, caused many side effects, including diarrhea, vomiting, and a loss of appetite. This was corrected by the procedure of soaking the gallbladders in rice vinegar for five years.
Another discovery was that shark gallbladder, when used singularly as treatment, was not sufficient in inhibiting the spread of cancer. It required the combination with four other ingredients: Stigam goci ( 西红花 ), Ligum aguilariae resinatum ( 沉香 ), Cornu saigae tartaricae ( 羚羊角 ), Radix notoginseng ( 参三七 ). In addition, Lord Liu discovered that deep flexor tendons in the Achilles tendon were crucial in the prevention of the spread of cancerous tumors.
Western Research
A steroid substance called squalamine is found in sharks. Squalamine is an aminosterol compound found in the bile-producing organs, such as the liver and gallbladder, of the dogfish shark.
Squalamine was first identified in July 1993 by Dr. Michael Zasloff. He was particularly interested in the immune systems of sharks. He worked with Karen Moore, a medical student, in a search for antibiotics that are produced in nature. This research was done initially to find a treatment for infection. Dr. Zasloff performed another study for the National Academy of Sciences in 1993 on the aminosterol antibiotic qualities of squalamine from sharks.1
In the 1990s, Dr. Henry Brem researched squalamine for characteristics that would help in the fight against breast cancer. Dr. Brem found that squalamine affected blood vessel proliferation (angiogenesis), the process by which tumors nourished themselves by creating circulatory paths.2
Another team of scientists led by Dr. Richard Pietras of the University of California, Los Angeles, was working to isolate the traits of squalamine. This team found that squalamine was antiangiogenic in ovarian cancer xenografts and that the compound had the ability to enhance the cytotoxic effects of cisplatin on ovarian cancer cells.3 This finding revealed that squalamine had the ability to inhibit the growth of blood vessels in an ovarian tumor and to render the cancer cells more susceptible to the chemotherapy drug cisplatin.
Many more studies have been performed to identify the chemical benefits of squalamine, as interest in this animal product has become heighted by the recognition of its potential importance. In 2001, the Lombardi Cancer Center of the Georgetown University Medical Centre in Washington, D.C., and Magainin Pharmaceuticals (currently Genaera Corporation, in Plymouth Meeting, Pennsylvania) performed a “phase I and pharmacokinetic study of squalamine, a novel antiangiogenic agent, in patients with advanced cancers.”6
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